Hematopoietic differentiation persists in human iPSCs defective in de novo DNA methylation

Background DNA methylation is involved in the epigenetic regulation of gene expression during developmental processes and is primarily established by the DNA methyltransferase 3A (DNMT3A) and 3B (DNMT3B). DNMT3A is one of the most frequently mutated genes in clonal hematopoiesis and leukemia, indicating that it plays a crucial role for hematopoietic differentiation. However, the functional relevance of Dnmt3a for hematopoietic differentiation and hematological malignancies has mostly been analyzed in mice, with the specific role for human hematopoiesis remaining elusive. In this study, we therefore investigated if DNMT3A is essential for hematopoietic differentiation of human induced pluripotent stem cells (iPSCs). Results We generated iPSC lines with knockout of either exon 2, 19, or 23 and analyzed the impact of different DNMT3A exon knockouts on directed differentiation toward mesenchymal and hematopoietic lineages. Exon 19−/− and 23−/− lines displayed an almost entire absence of de novo DNA methylation during mesenchymal and hematopoietic differentiation. Yet, differentiation efficiency was only slightly reduced in exon 19−/− and rather increased in exon 23−/− lines, while there was no significant impact on gene expression in hematopoietic progenitors (iHPCs). Notably, DNMT3A−/− iHPCs recapitulate some DNA methylation patterns of acute myeloid leukemia (AML) with DNMT3A mutations. Furthermore, multicolor genetic barcoding revealed growth advantage of exon 23−/− iHPCs in a syngeneic competitive differentiation assay. Conclusions Our results demonstrate that iPSCs with homozygous knockout of different exons of DNMT3A remain capable of mesenchymal and hematopoietic differentiation—and exon 23−/− iHPCs even gained growth advantage—despite loss of almost the entire de novo DNA methylation. Partial recapitulation of DNA methylation patterns of AML with DNMT3A mutations by our DNMT3A knockout iHPCs indicates that our model system can help to elucidate mechanisms of clonal hematopoiesis. Supplementary Information The online version contains supplementary material available at 10.1186/s12915-022-01343-x.

important in later development [2]. DNMT3A has five different protein-coding splice variants which might have different functional roles and are spliced in tissue-and disease-specific manner [3,4]. So far, it is largely unclear how DNAm is targeted to specific sites in the genome.
DNMT3A seems to be of particular relevance for hematopoietic differentiation, since it frequently reveals heterozygous mutations in acute myeloid leukemia (AML) and other hematological malignancies [5,6]. In AML, about 65% of the DNMT3A mutations are located in the hotspot R882 in exon 23 [7,8]. This mutation is believed to reduce enzyme activity by blocking its ability to form active tetramers with the wildtype form [9] and to induce aberrant DNAm patterns due to conformational changes in the enzyme [8]. Furthermore, DNMT3A holds the most frequent driver mutations in clonal hematopoiesis of indeterminate potential (CHIP) [10].
The functional relevance of Dnmt3a was investigated in homozygous knockout mice already more than 20 years ago, demonstrating that development remained possible despite undersize at birth and death at about 4 weeks of age [2]. More recent analysis of murine Dnmt3a knockout models revealed an increased self-renewal capacity of hematopoietic stem cells (HSCs) with reduced differentiation capacity [11]. Heterozygous knockouts had only a subtle effect on methylation in mice with a bias for myeloid differentiation and higher propensity for malignant transformation [12]. Other studies reported that knocking out Dnmt3a in HSCs and transplanting them into mice lead to development of a wide range of myeloid and lymphoid malignancies [13][14][15].
In contrast, relatively few studies addressed the consequences of DNMT3A knockout in human cells. In human embryonic stem cells, no immediate negative effects of DNMT3A −/− could be observed on downstream differentiation, although global loss of DNA methylation was observed after many passages with focal areas of hypermethylation [16]. Recently, it has been demonstrated that human-induced pluripotent stem cells (iPSCs) with knockout of DNMT3A can be differentiated into cardiomyocytes with similar efficiency as wildtype controls [17]. However, these iPSC-derived cardiomyocytes showed global hypomethylation with differences in contractile behavior and an aberrant activation of glucose and lipid metabolism [17].
In this study, we investigated the functional relevance of DNMT3A for hematopoietic differentiation of human iPSCs. To this end, we generated iPSC lines with knockout of exon 2, which contains a start codon for transcripts 1, 3, and 4. Alternatively, we removed exon 19, which has also been targeted in the abovementioned studies, or exon 23 that comprises the frequently mutated hotspot R882. We then analyzed the effect on growth and differentiation of iPSCs toward mesenchymal and hematopoietic lineages. We found only a moderate impact on differentiation with no significant changes in gene expression despite severe impact on de novo DNA methylation. Additionally, exon 23 knockout clones gained growth advantage over wildtype cells. Thus, de novo DNA methylation during early differentiation seems to be dependent on DNMT3A and disruption of the gene may be involved in competitive clonal advantages.

Knockout of DNMT3A exons does not evoke phenotypic changes in human iPSCs
To generate knockouts of different DNMT3A exons, we used iPSC lines from three donors for CRIPSR/Cas9n targeting of the intron/exon boundaries of exon 2 and exon 19, or excision of exon 23 ( Fig. 1a; Additional file 1: Fig. S1). For exon 2, we achieved a homozygous truncation of DNMT3A for donor 1, due to a new in frame start codon in exon 3, and a heterozygous knockout for donor 2. Furthermore, we generated two homozygous knockout lines for exon 19 and two homozygous knockout lines for exon 23, as determined by Western blot analysis ( Fig. 1b; Additional file 1: Fig. S2). These results were further substantiated by quantitative RT-PCR (Fig. 1c).
DNMT3A knockouts did not reveal negative effects on growth and differentiation of iPSCs. All clones showed typical iPSC-like morphology (Fig. 1d), expressed the pluripotency markers OCT3/4 and TRA1-60, and were able to differentiate toward endodermal, mesodermal, and ectodermal lineages (Additional file 1: Fig. S3a, b). To assess the impact of DNMT3A loss on DNA methylation, we used the Infinium Methylation EPIC BeadChip technology. Multidimensional scaling (MDS) indicated that DNAm profiles were similar in wildtype and DNMT3A knockout iPSC lines (Additional file 1: Fig. S3c). Pairwise comparison showed that only few CpGs had a clear conversion of DNAm levels (at least 50% gain or loss in DNAm) upon knockout, while there was no systematic off-set in DNAm levels (Fig. 1e, Additional file 2: Tab. S1). Taken together, we were able to successfully knock out three different exons of DNMT3A in human iPSCs and this did not evoke pronounced effects on DNAm levels while in a pluripotent cell state.

DNMT3A knockouts impair de novo DNAm in iPSC-derived mesenchymal cells
To test the impact of DNMT3A knockouts on directed differentiation, we initially differentiated all iPSC lines toward mesenchymal stromal cells (iMSCs) that constitute an important part of the hematopoietic niche. After 5 weeks, differentiated cells of all iPSC lines revealed typical fibroblastoid morphology (Fig. 2a) and many cells upregulated immunophenotypic markers for MSCs, albeit less pronounced for CD105 and CD73 in exon 19 −/− clones (Fig. 2b, Additional file 1: Fig. S4a). The differentiation of wildtype iPSCs toward iMSCs was associated with distinct DNAm changes of more than 50% at many CpGs: wildtype iMSCs gained DNAm at 2135 CpGs and lost methylation at 6700 CpGs ( Fig. 2c; Additional file 3: Tab. S2). Notably, hypermethylated CpGs were enriched in promoter regions of genes in functional categories associated with mesenchymal differentiation, such as skeletal system development, osteoblast development, and cartilage development, indicating that de novo DNAm during iMSC differentiation is indeed lineage specific (Additional file 1: Fig. S4b). A similar number of differentially methylated CpGs were observed for the exon 2 −/− clone (2509 hyper-and 9771 hypomethylated CpGs) and most of them were overlapping with the DNAm changes of wildtype clones (Additional file 1: Fig.  S4c). This might be anticipated, due to a new start codon in exon 3 maintaining the functional MTase-domain. In contrast, exon 19 −/− and exon 23 −/− clones hardly gained DNAm during differentiation toward iMSCs (only 31 and 32 CpGs, respectively), while hypomethylation followed a similar pattern as in wildtype iPSCs (10424 and 5857 CpGs, respectively). In fact, there was a very high overlap of hypomethylated CpGs in wildtype, exon 2 −/− , exon 19 −/− , and exon 23 −/− cells (Additional file 1: Fig.  S4c). Our results demonstrate that despite similarities in the molecular phenotype of iMSCs there was hardly any de novo DNAm during differentiation of iPSCs with To estimate if proliferation might be decreased in the exon 19 −/− clones that produced in tendency less hematopoietic cells, we analyzed expression of cell cycle-related genes and observed decreased expression of G2M and S phase genes in exon 19 −/− iHPCs, although differences in gene expression were not significant (Additional file 1: Fig. S5e). Overall, all iPSC lines were capable of differentiating toward hematopoietic lineage, but the propensity was reduced in exon 19 −/− as compared to wildtype or exon 23 −/− clones.
The differences in hematopoietic differentiation between exon 19 −/− and exon 23 −/− clones might be attributed to different DNA methylation profiles. Overall, only 110 CpGs were more than 50% hypomethylated c Absolute count of harvested iHPCs per well. Statistics were calculated with 1-way ANOVA and Tukey's post hoc test; n = 7 for wildtype (WT), n = 4 for each knockout (*P < 0.05; mean ± SD). d Surface marker expression of hematopoietic progenitors. Statistics were calculated with 2-way ANOVA and Tukey's post hoc test; n = 7 for WT, n = 4 for each knockout (*P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001; mean ± SD). e Total number of colonies formed in CFU assays. Statistics were calculated with 1-way ANOVA and Tukey's post hoc test; n = 7 for WT, n = 4 for each knockout (*P < 0.05; mean ± SD). f Scatter plots show β-values for all measured CpG sites (grey) when comparing the mean of wildtype iHPCs, exon 19 −/− iHPCs or exon 23 −/− iHPCs with their respective iPSC counterparts. Hypermethylated CpGs are depicted in red, hypomethylated CpGs in blue with delta mean β-value >0.5 or <−0.5. CpG sites associated with promotor regions are shown without transparency. g A deconvolution algorithm [18] was used to estimate the composition of different mature hematopoietic cell types based on the DNAm profiles of our iHPCs, those of Nishizawa et al. [19] and of our previously published iHPC data [20]. h Gene expression changes compared between WT iHPCs and exon 19 −/− or exon 23 −/− iHPCs depicted as log 2 fold change against the mean of normalized counts of all samples regarding sequencing depth (=base mean). Significantly differentially expressed genes are highlighted and 353 CpGs were more than 50% hypermethylated in exon 23 −/− versus exon 19 −/− lines. Very similar results were observed when we only compared the two samples of donor 1, to prevent impact of different genetic backgrounds (Additional file 1: Fig. S7a, b).
To estimate if DNMT3A knockouts affected differentiation toward specific hematopoietic lineages, we used a deconvolution algorithm to determine the composition of hematopoietic subsets based on DNAm profiles [18] (Fig. 3g). DNAm profiles of wildtype iHPCs indicated multilineage differentiation, similar to previously published iHPC profiles [19,20]. Despite the impact of exon 19 −/− and exon 23 −/− on de novo DNAm, estimates for cellular composition were similar between wildtype and knockout iHPCs. This might be attributed to the finding that cell-type specific DNAm patterns of such signatures are rather hypomethylated [18,21], and the hypomethylation was largely maintained in DNMT3A knockouts. Alternatively, we focused on individual CpGs that are specifically hypomethylated in granulocytes, monocytes, B cells, CD4, CD8, or NK cells [20,22]. Overall, DNAm patterns at these lineage-specific sites were similar in wildtype and DNMT3A knockout iHPCs (Additional file 1: Fig. S6c).
To investigate if impaired de novo DNAm in DNMT3A knockouts was also reflected on transcriptomic level, we performed RNA-sequencing analysis. Unexpectedly, there were no significant differences in wildtype versus either exon 19 −/− or exon 23 −/− clones (Wald test; P < 0.05; Fig. 3h; Additional file 6: Tab. S5). Furthermore, even those genes that showed a difference of more than 50% on DNAm levels did not reveal a clear tendency for differential expression, even if we focused on DNAm changes in the promoter regions (Additional file 1: Fig.  S6d). When we compared differential gene expression of iHPCs between exon 19 −/− or exon 23 −/− clones, only three genes reached significance (Hemoglobin Subunit Delta (HBD), V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1), and Gamma-Aminobutyric Acid Type A Receptor Subunit Pi (GABRP; Fig. 3h). Taken together, loss of de novo methylation in exon 19 −/− and exon 23 −/− clones was not clearly reflected in differential gene expression.

Impact of DNMT3A knockouts on specific DNAm changes
To gain better insight into how global DNAm patterns change in iMSCs and iHPCs, we performed a principal component analysis (PCA). Despite the marked block of de novo DNAm, there are still cell-type specific changes, which can be particularly attributed to focal hypomethylation (Fig. 4a). Notably, many pluripotency-associated DNAm patterns, which are usually lost during differentiation, were maintained in iMSCs and iHPCs of exon 19 −/− and exon 23 −/− lines. This is exemplified by the Epi-Pluri-Score analysis, which resembles a targeted epigenetic signature to discern pluripotent and non-pluripotent cells [23], and methylation in the POU5F1 promoter ( Fig. 4b; Additional file 1: Fig. S8a, b). We have previously described that age-associated DNAm patterns are reversed during reprogramming into iPSCs [24] and all of our iPSC, iMSC, and iHPC lines maintained this rejuvenated epigenetic phenotype (Additional file 1: Fig. S8c).
Recently, it has been reported that genomic regions with low DNA methylation, termed long DNA methylation canyons, can form large loops connecting anchor loci of inter-chromosomal interaction [25]. Dnmt3a knockout mice demonstrated erosion of methylation canyons near developmental regulatory genes, like HOX clusters, or in association with HSC self-renewal genes [11]. Such analysis of large hypomethylated regions is better suited for whole genome bisulfite sequencing data, but we could also detect these canyons in our EPIC Bead-Chip data. In analogy to the previous studies in mice, particularly the canyon borders became hypomethylated in exon 19 −/− and exon 23 −/− knockout lines, as exemplarily depicted for the canyons in PAX6 and GATA2 (Additional file 1: Fig. S8d).

DNAm associated with DNMT3A mutations in AML is recapitulated in knockout iHPCs
To better understand if DNAm changes of our DNMT3A knockout iPSC clones are related to aberrant DNAm patterns in AML patients with DNMT3A mutations, we utilized 134 DNAm datasets of AML patients from The Cancer Genome Atlas (TCGA) without mutation in DNMT3A (n = 101), with R882 mutations (n = 18), or with other DNMT3A mutations (n = 15). AML patients with the R882 mutations revealed global hypomethylation, as compared to AML patients without DNMT3A mutation (Fig. 5a): 7976 CpGs were more than 20% hypomethylated in R882 patients, whereas only 10 CpGs were 20% higher methylated. Notably, these hypomethylated CpGs showed on average also lower DNAm in our exon 19 −/− or exon 23 −/− iHPCs versus wildtype iHPCs (P < 10 −5 as compared to all other CpGs for both comparisons; Fig. 5b, c). When we compared DNAm patterns in AML patients with other DNMT3A mutations to AML patients without DNMT3A mutations, the general hypomethylation was much less pronounced and only 896 CpGs revealed 20% lower DNAm with DNMT3A mutation, while 181 CpGs had at least 20% higher DNAm levels (Fig. 5d). Again, these hypomethylated CpGs were also hypomethylated in in our exon 19 −/− or exon 23 −/− iHPCs versus wildtype iHPCs (P < 10 −5 for both comparisons, Fig. 5e, f ). Thus, the DNAm patterns in AML patients that are related to DNMT3A mutations (R882 as well as other mutations) are partly recapitulated by our iPSC knockout models.

Exon 23 knockout shows growth advantage during competitive hematopoietic differentiation
We next wanted to better understand subclonal development during hematopoietic differentiation of iPSCs with and without DNMT3A knockouts. To this end, we transduced wildtype, exon 19 −/− , or exon 23 −/− clones with lentiviral vectors containing unique molecular identifiers and the fluorophores Venus, Cerulean, or  (Fig. 6a). The three syngeneic iPSC lines were then mixed with equal cell numbers and seeded on the microcontact-printed plates for EB formation (Fig. 6b). After hematopoietic differentiation, flow cytometric analysis demonstrated growth disadvantage of Cerulean labeled exon 19 −/− clones (Fig. 6c). Furthermore, we analyzed the fluorophore-specific genetic barcodes by amplicon deep-sequencing and exon 23 −/− cells had clear growth advantage with increasing fractions upon EB formation, iHPC production, and after additional long-term culture expansion for 28 days, whereas counts for exon 19 −/− cells decreased with time (Fig. 6d). These findings are in line with the higher iHPC numbers in both exon 23 knockouts, as indicated in Fig. 3c. However, exon 19 −/− clones did not reveal significant decline as compared to wildtype cells, even after 50 days in culture. Subsequently, the fractions of corresponding unique molecular identifier were tracked over the different time points demonstrating that hematopoietic differentiation of iPSCs is a multiclonal event (Fig. 6e). While some exon 23 −/− barcodes made up to 1.51% of all reads, there was no evidence for a clear oligo clonal composition after long-term expansion.

Discussion
So far, the sequel of losing DNMT3A activity for hematopoietic development has mostly been investigated in the murine system by knocking out exon 19 or exon 18-20 of Dnmt3a with the Cre/loxP system [26,27], or by targeting exon 19 with CRISPR/Cas9 [28]. Two groups established DNMT3A knockouts in human ESCs and iPSCs by targeting exon 19 [16,17], but they did not describe their hematopoietic differentiation potential. iPSCs are a valuable model system for hematopoietic differentiation, because they facilitate systematic and clonal analysis in homogenous cell preparations [29].
Our results demonstrate that iPSCs with knockout of DNMT3A remain capable of differentiating toward Fig. 6 Competitive growth advantage of exon 23 −/− cells during hematopoietic differentiation. a Syngeneic wildtype, exon 19 −/− and exon 23 −/− iPSCs were transduced with a lentiviral barcoding system and the fluorophores Venus, Cerulean, and mCherry, respectively. The schematic representation depicts competitive growth in co-culture during embryoid body (EB) formation, hematopoietic differentiation, and additional long-term culture (n = 3, all donor 1). b Representative phase contrast and fluorescence microscopic images of EBs with mixed populations at day −4 (day 3 of EB growth). c Flow cytometry analysis of iHPCs after 15 days of hematopoietic differentiation (only cells with a clear fluorescent signal were considered). n = 3 (mean ± SD). d Alternatively, we analyzed the fluorophore-specific genetic barcodes by amplicon sequencing after mixing of the iPSCs (d-7), in EBs (d0), in iHPCs (d15), and after additional long-term expansion (d43). n = 3 (mean ± SD). e Area plots show clonal growth during differentiation and expansion of the three replicates. The graded colors depict corresponding unique molecular identifiers of the lentiviral barcoding. All statistics were performed with 1-way ANOVA and Tukey's post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 hematopoietic lineages. We did not directly determine proliferation rates, but there may be evidence that exon 19 −/− lines have in tendency a slightly lower proliferation than wildtype clones, as suggested by expression of cell cycle-related genes and multicolor barcode analysis. Despite the pronounced differences in DNAm profiles of wildtype and DNMT3A −/− clones, we did not find any significant differences in gene expression, indicating that de novo DNA methylation is not essential for differentiation into iHPCs. However, it is possible that deeper sequencing or more replicates would provide a more detailed view on the gene expression changes. It also needs to be considered that DNAm patterns of wildtype and knockout clone-derived iPSCs, iHPCs, and iMSCs still clustered together, probably due to cell-type specific hypomethylation, which may be functionally more relevant. Furthermore, other epigenetic modifications, such as histone modifications and chromatin conformation, may be primary drivers of directed cellular differentiation.
There are many different site-specific DNMT3A mutations in leukemia and CHIP [5,7], and therefore, it might be anticipated that targeting of specific exons is of functional relevance. Exon 2 mutations in AML have been described before but its functional impact remains unclear because this exon is not associated with a functional protein domain [30,31]. Mutations in exon 19 are highly abundant and may result in a functional loss [32,33]. Exon 19 encodes parts of the methyltransferase domain and can form hydrogen bonds with DNA for its binding and stabilization during methylation activity [34]. More research has been performed for mutations in exon 23, particularly the hotspot mutation R882, which often results in loss of DNAm [8,32]. This region is also involved in the methyltransferase domain and it is essential for interacting with the DNA backbone during DNMT3A-DNMT3A tetramer formation [34]. Our results support the notion that there may be functional differences between the different DNMT3A knockouts, but it needs to be considered that for each exon only two knockout lines have been generated and there is notorious variation in the differentiation potential of such cell lines. Furthermore, there were hardly differences in the DNA methylation profiles of iHPCs with exon 19 −/− and exon 23 −/− . Additional research is therefore necessary to ultimately answer the question how aberrant splicing and specific mutations affect hematopoietic differentiation.
Only very few CpGs consistently gained DNAm in our exon 19 −/− and exon 23 −/− clones. They revealed a highly significant overlap in iMSCs and iHPCs and were associated with pluripotency-and developmentassociated transcription factor binding sites. Whether a pluripotency-associated regulatory mechanism might be involved in the residual de novo DNA methylation during differentiation needs to be further tested. In primary hematopoiesis, Dnmt3a has been shown to be a critical participant in the epigenetic silencing of HSC regulatory genes, thereby enabling efficient differentiation [26]. In contrast, Dnmt3b is also highly abundant in HSCs, but rather in a catalytically inactivated form with just some residual activity [2,35]. The de novo methylation of these few CpGs might therefore be attributed to DNMT3B.
Acute myeloid leukemia is associated with characteristic aberrations in the DNA methylation pattern [36]. Patient samples with the hotspot mutation R882 revealed prominent hypomethylation compared to other DNMT3A mutations, as described before [9]. Our results demonstrate that iPSC-derived model iHPCs can recapitulate some epigenetic effects of R882-mutated and even in non-R882-mutated AML. Thus, aspects of AMLrelated DNAm patterns can be modeled by our system, which may be useful for further mechanistic analysis.
Lastly, we investigated if our iPSC model system also reveals subclonal dominance of DNMT3A knockouts in a competitive differentiation assay with long-term culture. In fact, exon 23 −/− iHPCs revealed growth advantage over wildtype and exon 19 −/− lines but there was no evidence for dominant subclones. In contrast, the lentiviral barcoding system showed dominant subclones in iMSCs, even without modifications in DNMT3A [37]. It is conceivable that differentiation of iHPCs is more homogeneous than iMSCs or that the effects of DNMT3A knockouts become only apparent after even longer culturing periods. Either way, a moderate growth advantage of clones with specific aberrations in DNMT3A might be a functional correlate to malignant transformation.

Conclusions
Hematopoietic differentiation is hardly affected in iPSCs with homozygous knockout of exon 19 or 23, despite almost entire absence of de novo methylation. This was unexpected given the functional relevance of DNAm during differentiation. It will therefore be important to gain better insight into cell-type specific hypomethylation and the interplay with other epigenetic modifications. Our model system can help to get a better understanding on the relevance of DNMT3A during hematopoietic development and malignant transformation.

iPSC lines, ethics approval, and consent to participate
iPSCs of donor 1 and donor 2 were reprogrammed from MSC preparations with episomal plasmids and characterized, as described before [38]. iPSCs of donor 3 were generated from KIT+ cells from bone marrow aspirates of a mastocytosis patient, with the CytoTune-iPS Sendai Reprogramming Kit (Thermo Fisher Scientific, Waltham, MA, USA). This line was also carefully characterized and revealed a KRAS mutation A146T. All samples were taken after informed and written consent and the study was approved by the Ethic Committee of the Use of Human Subjects at the University of Aachen (permit numbers: EK128/09 and EK206/09, respectively). iPSCs were cultured on tissue culture plastic coated either with vitronectin (0.5 mg/cm 2 ; Stemcell Technologies, Vancouver, Canada) or Matrigel Matrix (Corning, Corning, NY, USA) in StemMACS iPS-Brew XF (Miltenyi Biotec, Bergisch Gladbach, Germany) with 100 U/mL penicillin and 100 μg/mL streptomycin. Pluripotency was validated by Epi-Pluri-Score (Cygenia GmbH, Aachen, Germany) [23].

CRISPR/Cas9n knockout of DNMT3A
Knockouts of exon 2, 19, and 23 of DNMT3A were generated with CRISPR/Cas9n double nicking approach [39]. Two pairs of gRNAs were designed with the help of the CRISPOR tool [40] (Additional file 1: Fig. S1a & Tab. S6). Intron/exon boundaries of exon 2 and exon 19 were targeted in order to achieve altered splicing via exon skipping. Complete excision of exon 23 was performed as this exon did not allow for design of effective gRNAs for exon/intron boundary targeting. gRNA primers were individually ligated into a variant of the pX335 vector (Addgene #42335) additionally containing the reporter protein GFP and a puromycin selection cassette [41,42]. Transfection of 10 6 iPSCs with the four gRNA plasmids (2.5 μg each) was performed with the NEON transfection system kit (Thermo Fisher Scientific) and after 1 day transfected cells were selected with 0.4 μg/mL puromycin for 48 h. Single colonies were picked and screened for DNMT3A deletions by PCR and Sanger sequencing (primers: Additional file 1: Tab. S7). Knockout was further confirmed with semi-quantitative real-time PCR (primers: Additional file 1: Tab. S8) and Western blots; pluripotency was tested with immunofluorescence and trilineage differentiation assays (qPCR primers for trilineage analysis: Additional file 1: Tab. S9) as described in Additional file 1.

Differentiation of iPSCs into the mesenchymal lineage
For iMSC generation, iPSC medium was switched to MSC medium, consisting of Dulbecco's modified Eagle's medium-low glucose (Thermo Fisher Scientific) supplemented with 10% human platelet lysate, and 5 U/mL heparin (Rotexmedica, Trittau, Germany) for 35 days, as described before [43]. Surface marker expression was measured with flow cytometry (Additional file 1). We have also analyzed three lineage differentiation potential of iMSCs as described before [43], but due to high variation between cell lines and replicas a reliable quantitative comparison was not feasible.

DNA methylation analysis
Genomic DNA was isolated with the NucleoSpin Tissue kit (Macherey Nagel) and subsequently bisulfite converted and hybridized to the Infinium MethylationEPIC BeadChip (Illumina, San Diego, California, USA) at Life and Brain GmbH (Bonn, Germany). Data was preprocessed with the Bioconductor Illumina Minfi package for R [46,47] and normalized with ssnoob. CpGs on X and Y chromosomes, SNPs, and cross-reactive sites were excluded, resulting in 741896 CpGs. For comparison with other datasets (iHPC: GSE60811 [19,48] and GSE119079 [20,49]; whole blood, granulocytes, B cells, CD4+ T cells, CD8+ T cells, NK cells, monocytes: all GSE35069 [50,51]; CD34+ cells isolated from human cord blood: GSE40799 [52,53]; primary MSCs: GSE113527 [54,55]; AML data obtained from The Cancer Genome Atlas [56]), we further focused on CpGs that were presented by the Illumina Infinium HumanMethylation450 Bead-Chip and the Infinium MethylationEPIC BeadChip. Principal component analysis and multidimensional scaling plots were prepared in R using the ggplot2 package version 3.3.3 [57]. Heatmaps were generated with the MultiExperiment Viewer (MeV; version 4.9.0). Gene ontology analysis was performed on genes with differentially methylated CpGs in the promoter region (located in TSS1500, TSS200, and 5′UTR) in R with the missMethyl package [58]. Categories comprising more than 1000 genes were not considered and similar categories are only listed once. Venn diagrams were prepared with the Inter-actiVenn tool [59]. Motif analysis was performed with the Hypergeometric Optimization of Motif EnRichment (HOMER) suite [60] with a 100 bp frame around hypermethylated CpGs. Canyons were defined as 30 neighboring CpG sites within 3 kb and an average β-value below 0.15. Epigenetic age was predicted using Horvath's skin and blood clock [61].

Gene expression analysis
For transcriptomic analysis, total RNA was isolated from iHPCs at day 16 of differentiation with the NucleoSpin RNA Plus kit (Macherey Nagel). mRNA was prepared with the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Vienna, Austria) and sequenced with 10 million 50 bp single-end reads on the HiSeq 2500v4 (Illumina) at Life and Brain GmbH. Raw data underwent quality control with Trim Galore and sequences were aligned to the human genome (hg38) by bowtie2 [62] aligner with default parameters. Differential expression analysis was done by DESeq2 [63] with the adjusted p-value threshold of 0.05. Scatter plots were generated with ggplot2 [57] in R.

MiSeq analysis of barcoded cells
MiSeq was performed as described in detail before [37]. In brief, DNA was isolated with the NucleoSpin Tissue kit (Macherey Nagel), barcodes were amplified, tagged with Illumina sample barcodes, samples were pooled equimolarly, and a 4 nM library was prepared for sequencing with a 50% spike in of PhiX Control DNA and the MiSeq Reagent Kit v2 (all from Illumina). Libraries were sequenced in 250-bp paired-end mode on an Illumina MiSeq Benchtop Sequencer. Raw data was analyzed with Python. Unique molecular identifiers of the RGB-vectors were extracted from FastQ files and grouped according to color-specific sequences resembling the different cell lines. Two mismatches were allowed for grouping of these color-specific identifiers. Finally, directional clustering from UMI-tools [66] was used to specify unique molecular identifiers for each color-group. Area plots were prepared with ggplot2 in R.

Statistics and reproducibility
All experiments were performed with three wildtype iPSC lines and two knockout iPSC lines for DNMT3A exon 2, 19, and 23. Genetic barcoding experiments were performed with the syngeneic iPSC clones of donor 1 (corresponding wildtype, exon 19 knockout, and exon 23 knockout) in three technical replicates. Data is provided as mean with standard deviation (SD). Significance of flow cytometry data was calculated by 2-way ANOVA with Tukey's post hoc test. One-way ANOVA with Tukey's post hoc test was used for iHPC counts, CFU colonies, for comparison of methylation data with AML patients, and for flow cytometry data of RGB cells (GraphPad version 9.1.1.). Significantly differentially expressed genes were tested in R with DESeq2 using the default Wald test. Significance of overlapping hypermethylated CpGs within iMSCs and iHPCs of exon 19 and exon 23 knockout clones was calculated in R with the gmp package via hypergeometric distribution.